Development of a Wound-Healing Protocol for In Vitro Evaluation of Urothelial Cell Growth

Urethral healing is plagued by strictures, impacting quality of life and medical costs. Various growth factors (GFs) have shown promise as therapeutic approaches to improve healing, but there no protocol for in vitro comparison between GFs. This study focuses the development a biomimetic urothelial assay designed mimic early vivo followed an evaluation cell response Methods: Wound-healing assays were developed with human cells used compared six GFs (EGF, FGF-2, IGF-1, PDGF, TGF-β1, VEGF) at three concentrations (1 ng/mL, 10 100 ng/mL) over 48 h period. A commercial GF-containing medium TGF-α, KGF, Extract P) GF-free controls. Results: There was statistically significant increase IGF-1 ng/mL both controls (p < 0.05). EGF all control Conclusion: shows clinically relevant wound-healing evaluate growth. It first compare future use reconstructive techniques urethral healing.